InOut Biome, LLC Gut microbiome sampling

Ingestible Capsule

Mechanical - Single Use

A New Approach to Studying the Hidden Microbiome

Most microbiome studies rely on fecal samples—even though these reflect only the final stage of digestion. Many microbial communities, metabolites, enzymes, and molecular signals active in the upper gut do not survive the transition to stool.

InOut Biome™ is developing an ingestible sampling capsule for research use in humans and animals. The goal is to provide practical access to localized intestinal environments—capturing molecular signatures directly at the site of activity.

Why Localized Sampling Matters

Important biological processes occur upstream of the colon, including:

• Microbial co-occurrence and functional interactions
• pH-sensitive and oxygen-sensitive pathways
• Enzyme activity and metabolic transformation
• Region-specific microbial populations
• Early biochemical events not reflected in fecal material

These signals are often obscured or lost before reaching stool. Localized sampling allows them to be studied more directly.

THe Inout sampling Capsule

The capsule is an early-stage research platform designed to support scientific investigations of localized intestinal content.

Prototype features include:
• Targeted uptake of site-specific intestinal fluid
• Immediate activation of stabilization chemistry
• Formation of a low-oxygen internal environment
• Compact mechanical design suitable for ingestible research formats
• Modular adaptation for human or animal use
• A potential 7-day sample stability window, pending further study

Current development focuses on sampling behavior, internal conditions, and compatibility with sequencing and metabolomics workflows.

What We Aim to Preserve

Microbial Signatures

• Bacteria and archaea
• Virome and fungal components
• Functional gene pathways
• Microbial co-occurrence patterns

Sequencing compatibility:
• Shotgun metagenomics
• NGS (next-generation sequencing)
• Virome and fungal sequencing
• 16S rRNA sequencing

Metabolites

Compatible with:

• LC-MS (liquid chromatography–mass spectrometry)
• GC-MS (gas chromatography–mass spectrometry)
• NMR (nuclear magnetic resonance spectroscopy)

Biochemical Signals

• Enzymes and intermediates
• Peptides and small molecules
• pH-sensitive and redox-sensitive environments

Performance studies are ongoing.

Research Applications

Human Research

The platform is being developed to support studies of:

• Small intestine and upper GI biology
• Metabolite and enzyme dynamics
• Microbial pathways and networks
• Regional differences along the gut
• Sequencing-based microbiome and functional gene analysis

Animal Research

Research use is supported for:
• Dogs
• Swine
• Other mammals

Applications may include nutrition, digestive biology, host–microbe interactions, and multi-omics investigations.

InOut Biome, LLC Gut microbiome sampling

A New Approach to Studying the Hidden Microbiome

A New Approach to Studying the Hidden Microbiome

Why Localized Sampling Matters

THe Inout sampling Capsule

What We Aim to Preserve

Microbial Signatures

Metabolites

Biochemical Signals

Research Applications

Human Research

Animal Research

Research-Focused Development

Thank you for your interest. Contact us at: info@inoutbiome.com